• 新闻中心
  • 集团新闻
  • 行业动态
  • 视频中心
  • 技术中心
  • 技术团队
  • 技术成果
  • 技术前沿
  • 双服务体系
  • 市场活动
  • 服务支持
  • 常见问题
  • 产业发展
  • 浓缩饲料
  • 预混合饲料
  • 配合饲料
  • 联系我们
  • 招贤纳士
  • 联系方式
  • 集团新闻
  • 行业动态
  • 视频中心
  • 技术团队
  • 技术服务团队
  • 技术品控团队
  • 技术成果
  • 技术前沿
  • 市场活动
  • 服务支持
  • 常见问题
  • 浓缩饲料
  • 预混合饲料
  • 配合饲料
  • 招贤纳士
  • 联系方式
  • 技术中心

    日粮VA影响断奶仔猪肠道干细胞,进而影响生长性能、肠道发育和功能

    发布单位:贝博官网登录

    查看次数:375

    时间:2020-03-24
           VA是断奶仔猪的关键营养之一,它在维持上皮细胞正常形态、发育上起着重要作用。前人的研究表明添加VA可以改善机体肠道屏障功能。因此,我们假设日粮中添加VA可以通过调节肠道干细胞影响肠道功能。

           试验选用32头21d断奶仔猪((约克夏×长白)×杜洛克),平均重8.34±0.13kg,随机分至4个处理组,VA水平分别为2(对照组)、4、8、16mg/kg。试验期14d。断奶仔猪自由采食和饮水。

           试验第8~14d仔猪日增重和肉料比随VA添加水平的升高而线性提高(P=0.02、P=0.005)。16mg/kg VA组空肠Lgr5+基因表达显著提高(P=0.012)。4 mg/kg VA组的空肠绒毛高度和绒毛表面积显著提高(P值分别为0.027和0.035)。4、8mg/kg VA组隐窝深度显著增加(二次方程式,P=0.043),16mg/kg VA组绒毛高度:隐窝深度显著提高(二次方程式,P=0.015)。当进一步补充4mg/kg VA时,麦芽糖酶、蔗糖酶和碱性磷酸酶活性显著提高(P=0.032、0.041、0.024)。2mg/kg VA组的Slc2a2 mRNA丰度显著提高(线性,P=0.024)。此外,VA及其代谢产物(视黄酸)显着降低了仔猪肠道类器官的出芽率,每个类器官的出芽数以及嗜铬粒蛋白A和Muc2的表达(P <0.05)。与对照组相比,Spp1和Trop2的表达显著提高。

          这些结果表明VA可能会增加体外肠道干细胞的干性。本试验结果表明VA能通过调节空肠肠道干细胞影响断奶仔猪生长性能和肠道功能。

    Dietary vitamin A affects growth performance, intestinal development, and functions in weaned piglets by affecting intestinal stem cells

    Vitamin A (VA) is an important nutrient for weaning piglets. It plays a significant role in the normal formation, development, and maintenance of epithelial cells. Previous studies have shown that VA supplements could improve the host’s intestinal barrier function. Therefore, we hypothesized that VA supplements can affect intestinal function in weaned piglets by regulating intestinal stem cells. Thirty-two 21-d-old weaned [(Yorkshire × Landrace) × Duroc] piglets with an average weight of 8.34 ± 0.13 kg were randomly divided into 4 treatment groups, with 1) 2 mg/kg (control), 2) 4 mg/kg, 3) 8 mg/kg, and 4) 16 mg/kg doses of VA, respectively. The experiment lasted for 14 d. Weaned piglets were given ad libitum access to food and water during the test. The ADG (linear, P = 0.020) and G:F (linear, P = 0.005) of the piglets were found to increase significantly from days 8 to 14. The Lgr5+ gene expression (P = 0.012) in the jejunum mucosa of the 16 mg/kg VA group was increased. The jejunum villus height (P = 0.027) and villi surface area (P = 0.035) were significantly increased in the 4 mg/kg VA treatment group. The crypt depth increased significantly in the 4 and 8 mg/kg VA treatment groups (quadratic, P = 0.043), and the ratios of villus height to crypt depth significantly increased in the 16 mg/kg VA group (quadratic, P = 0.015). The maltase (P = 0.032), sucrose (P = 0.041), and alkaline phosphatase activity (linear, P = 0.024) were significantly increased when further supplemented with 4 mg/kg VA. Slc2a2 mRNA abundance was significantly increased in the 2 mg/kg VA group (linear, P = 0.024). Moreover, the budding rates, buddings number per organoid, and Chromogranin A and Muc2 expression of piglet intestinal organoids were significantly reduced (P < 0.05) by VA and its metabolites (retinoic acid). Compared with the control group, the expression of Spp1 and Trop2 increased. These results indicated that VA may increase the stemness of intestinal stem cell in vitro. This study suggested that VA could affect growth performance and intestinal function by regulating intestinal stem cells in the jejunum of weaned piglets.

    文章来源:猪营养国际论坛
    【免责声明】:文章来源于网络,我们对文中陈述观点判断保持中立,并不对文章观点负责。仅供读者参考。版权属于原作者。


  • 首 页

  • 联系我们

  • 二维码

  • 返回顶部